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Volume : IV, Issue : V, June - 2014

ISOLA TION AND CHARACTERIZA TION OF PHOSPHOBACTERIAL ISOLA TES FROM BHENDI RHIZOSPHERE SOILS OFCUDDALORE DISTRICT

Manikandan, S., K. Muthuselvam

By : Laxmi Book Publication

Abstract :

In the present study , an attempt was made to isolate and characterize the phosphobacterial isolates such as Bacillus sp. and Pseudomonas sp. from fifteen bhendi rhizosphere soil samples collected from Cuddalore district of T amil Nadu. The results showed that all rhizosphere soils harbored -1 phosphobacterial isolates such as Bacillus and Pseudomonas to the tune of 104 CFU g of rhizosphere soil sample. Pure culture of the above phosphobacterial isolates were prepared and further subjected to different characterization studies for their tentative identification. General and biochemical characterization studies clearly revealed that seven isolates of B. megaterium, three each isolates of B. subtilis and B. polymyxa and two isolates of B. cereus were found among the fifteen Bacillus rhizosphere soils. With regard to Pseudomonas population eight isolates of P . fluor escens, four isolates of P . putida and three isolates of P . striata were also obtained among the fifteen rhizosphere soils

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    Cite This Article :

    Manikandan, S., K. Muthuselvam(2014). ISOLA TION AND CHARACTERIZA TION OF PHOSPHOBACTERIAL ISOLA TES FROM BHENDI RHIZOSPHERE SOILS OFCUDDALORE DISTRICT. Indian Streams Research Journal, Vol. IV, Issue. V, http://isrj.org/UploadedData/4740.pdf

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    33. Adhikari, A., S. Dutta, S. Nandi, I. Bhattacharya, M. De Roy, G. Sarkar and T . Mandal. 2013. Antagonistic potentiality of native rhizobacterial isolates against root rot disease of okra, incited by Rhizoctonia solani. Afr. J. Agric. Res., 8(4): 405-412.
    34. Antoun, H., C. J. Beauchamp, N. Gousszrd, R. Chabat and R. Lalancle. 1998. Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: e? ect on radishes (Raphanus stativus L.). Plant Soil. 204: 57-67.
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    37. Antoun, H., C. J. Beauchamp, N. Gousszrd, R. Chabat and R. Lalancle. 1998. Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: e? ect on radishes (Raphanus stativus L.). Plant Soil. 204: 57-67.
    38. Anita Pandey , A., S. T . Shende and M. Singh. 1999. Azotobacter chroococcum seed inoculant for cotton (Gossypium hirsutum) - Effect of seed quality . Zentralbl. Microbiol., 144: 605-610.
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    63. Anita Pandey , A., S. T . Shende and M. Singh. 1999. Azotobacter chroococcum seed inoculant for cotton (Gossypium hirsutum) - Effect of seed quality . Zentralbl. Microbiol., 144: 605-610.
    64. Adhikari, A., S. Dutta, S. Nandi, I. Bhattacharya, M. De Roy, G. Sarkar and T . Mandal. 2013. Antagonistic potentiality of native rhizobacterial isolates against root rot disease of okra, incited by Rhizoctonia solani. Afr. J. Agric. Res., 8(4): 405-412.
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    67. Adhikari, A., S. Dutta, S. Nandi, I. Bhattacharya, M. De Roy, G. Sarkar and T . Mandal. 2013. Antagonistic potentiality of native rhizobacterial isolates against root rot disease of okra, incited by Rhizoctonia solani. Afr. J. Agric. Res., 8(4): 405-412.
    68. Anita Pandey , A., S. T . Shende and M. Singh. 1999. Azotobacter chroococcum seed inoculant for cotton (Gossypium hirsutum) - Effect of seed quality . Zentralbl. Microbiol., 144: 605-610.
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    79. Antoun, H., C. J. Beauchamp, N. Gousszrd, R. Chabat and R. Lalancle. 1998. Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: e? ect on radishes (Raphanus stativus L.). Plant Soil. 204: 57-67.
    80. Adhikari, A., S. Dutta, S. Nandi, I. Bhattacharya, M. De Roy, G. Sarkar and T . Mandal. 2013. Antagonistic potentiality of native rhizobacterial isolates against root rot disease of okra, incited by Rhizoctonia solani. Afr. J. Agric. Res., 8(4): 405-412.
    81. Adhikari, A., S. Dutta, S. Nandi, I. Bhattacharya, M. De Roy, G. Sarkar and T . Mandal. 2013. Antagonistic potentiality of native rhizobacterial isolates against root rot disease of okra, incited by Rhizoctonia solani. Afr. J. Agric. Res., 8(4): 405-412.
    82. Anita Pandey , A., S. T . Shende and M. Singh. 1999. Azotobacter chroococcum seed inoculant for cotton (Gossypium hirsutum) - Effect of seed quality . Zentralbl. Microbiol., 144: 605-610.
    83. Anita Pandey , A., S. T . Shende and M. Singh. 1999. Azotobacter chroococcum seed inoculant for cotton (Gossypium hirsutum) - Effect of seed quality . Zentralbl. Microbiol., 144: 605-610.
    84. Aneja, K. R. 1993. Experiments in Microbiology and Plant pathology , T issue culture and Mushroom cultivation.Wishwa Prakashan, New Delhi. p. 451.
    85. Adhikari, A., S. Dutta, S. Nandi, I. Bhattacharya, M. De Roy, G. Sarkar and T . Mandal. 2013. Antagonistic potentiality of native rhizobacterial isolates against root rot disease of okra, incited by Rhizoctonia solani. Afr. J. Agric. Res., 8(4): 405-412.
    86. Anita Pandey , A., S. T . Shende and M. Singh. 1999. Azotobacter chroococcum seed inoculant for cotton (Gossypium hirsutum) - Effect of seed quality . Zentralbl. Microbiol., 144: 605-610.
    87. Adhikari, A., S. Dutta, S. Nandi, I. Bhattacharya, M. De Roy, G. Sarkar and T . Mandal. 2013. Antagonistic potentiality of native rhizobacterial isolates against root rot disease of okra, incited by Rhizoctonia solani. Afr. J. Agric. Res., 8(4): 405-412.
    88. Anita Pandey , A., S. T . Shende and M. Singh. 1999. Azotobacter chroococcum seed inoculant for cotton (Gossypium hirsutum) - Effect of seed quality . Zentralbl. Microbiol., 144: 605-610.
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    91. Adhikari, A., S. Dutta, S. Nandi, I. Bhattacharya, M. De Roy, G. Sarkar and T . Mandal. 2013. Antagonistic potentiality of native rhizobacterial isolates against root rot disease of okra, incited by Rhizoctonia solani. Afr. J. Agric. Res., 8(4): 405-412.
    92. Antoun, H., C. J. Beauchamp, N. Gousszrd, R. Chabat and R. Lalancle. 1998. Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: e? ect on radishes (Raphanus stativus L.). Plant Soil. 204: 57-67.
    93. Anita Pandey , A., S. T . Shende and M. Singh. 1999. Azotobacter chroococcum seed inoculant for cotton (Gossypium hirsutum) - Effect of seed quality . Zentralbl. Microbiol., 144: 605-610.
    94. Adhikari, A., S. Dutta, S. Nandi, I. Bhattacharya, M. De Roy, G. Sarkar and T . Mandal. 2013. Antagonistic potentiality of native rhizobacterial isolates against root rot disease of okra, incited by Rhizoctonia solani. Afr. J. Agric. Res., 8(4): 405-412.
    95. Anita Pandey , A., S. T . Shende and M. Singh. 1999. Azotobacter chroococcum seed inoculant for cotton (Gossypium hirsutum) - Effect of seed quality . Zentralbl. Microbiol., 144: 605-610.
    96. Adhikari, A., S. Dutta, S. Nandi, I. Bhattacharya, M. De Roy, G. Sarkar and T . Mandal. 2013. Antagonistic potentiality of native rhizobacterial isolates against root rot disease of okra, incited by Rhizoctonia solani. Afr. J. Agric. Res., 8(4): 405-412.
    97. Allen, O. N. 1953. Experiments in Soil Bacteriology. Burgess Publ. Co., Minneapology Minnesota, U.S.A., p. 69-70.
    98. Anita Pandey , A., S. T . Shende and M. Singh. 1999. Azotobacter chroococcum seed inoculant for cotton (Gossypium hirsutum) - Effect of seed quality . Zentralbl. Microbiol., 144: 605-610.
    99. Allen, O. N. 1953. Experiments in Soil Bacteriology. Burgess Publ. Co., Minneapology Minnesota, U.S.A., p. 69-70.
    100. Aneja, K. R. 1993. Experiments in Microbiology and Plant pathology , T issue culture and Mushroom cultivation.Wishwa Prakashan, New Delhi. p. 451.
    101. Antoun, H., C. J. Beauchamp, N. Gousszrd, R. Chabat and R. Lalancle. 1998. Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: e? ect on radishes (Raphanus stativus L.). Plant Soil. 204: 57-67.
    102. Antoun, H., C. J. Beauchamp, N. Gousszrd, R. Chabat and R. Lalancle. 1998. Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: e? ect on radishes (Raphanus stativus L.). Plant Soil. 204: 57-67.
    103. Allen, O. N. 1953. Experiments in Soil Bacteriology. Burgess Publ. Co., Minneapology Minnesota, U.S.A., p. 69-70.
    104. Aneja, K. R. 1993. Experiments in Microbiology and Plant pathology , T issue culture and Mushroom cultivation.Wishwa Prakashan, New Delhi. p. 451.
    105. Antoun, H., C. J. Beauchamp, N. Gousszrd, R. Chabat and R. Lalancle. 1998. Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: e? ect on radishes (Raphanus stativus L.). Plant Soil. 204: 57-67.
    106. Allen, O. N. 1953. Experiments in Soil Bacteriology. Burgess Publ. Co., Minneapology Minnesota, U.S.A., p. 69-70.
    107. Aneja, K. R. 1993. Experiments in Microbiology and Plant pathology , T issue culture and Mushroom cultivation.Wishwa Prakashan, New Delhi. p. 451.
    108. Aneja, K. R. 1993. Experiments in Microbiology and Plant pathology , T issue culture and Mushroom cultivation.Wishwa Prakashan, New Delhi. p. 451.
    109. Aneja, K. R. 1993. Experiments in Microbiology and Plant pathology , T issue culture and Mushroom cultivation.Wishwa Prakashan, New Delhi. p. 451.
    110. Antoun, H., C. J. Beauchamp, N. Gousszrd, R. Chabat and R. Lalancle. 1998. Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: e? ect on radishes (Raphanus stativus L.). Plant Soil. 204: 57-67.
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